Fig 1: CCI-induced Lyn upregulation mainly in microglia of the ipsilateral spinal dorsal horn. (A) Double staining of Lyn and microglia activation marker IBA-1; (B) quantitative analysis of mean intensity of Lyn; (C) quantitative analysis of mean intensity of IBA-1; (D) number of positive cell co-expressing Lyn and IBA-1.
Fig 2: Lyn inhibitor Bafetinib intrathecally alleviated pain hyperalgesia in CCI mice. (A) Mechanical withdraw frequencies to 0.07 g after single intrathecal administration of different doses of Bafetinib on CCI mice; (B) mechanical withdraw frequencies to 0.4 g after single intrathecal administration of different doses of Bafetinib on CCI mice; (C) thermal pain thresholds after single intrathecal administration of different doses of Bafetinib on CCI mice; (D) mechanical withdraw frequencies to 0.07 g after continuous Bafetinib intrathecally on CCI mice; (E) mechanical withdraw frequencies to 0.4 g after continuous Bafetinib intrathecally on CCI mice; (F) Thermal pain thresholds after continuous Bafetinib intrathecally on CCI mice.
Fig 3: Lyn expression was also upregulated in cultured primary microglia under LPS or IFN-? stimulation. (A, D) Representative images of protein expressions of p-Lyn and Lyn of cultured primary microglia under LPS stimulation; (B, E) statistical analysis of protein expressions of p-Lyn and Lyn of cultured primary microglia; (C, F) mRNA level of Lyn of cultured primary microglia.
Fig 4: CCI led to pain hyperalgesia and upregulation of Lyn expression in spinal dorsal horn. (A) Mechanical withdraw frequencies to 0.07 g; (B) mechanical withdraw frequencies to 0.4 g; (C) thermal pain thresholds; (D) representative images of protein expressions of p-Lyn and Lyn of mice; (E) statistical analysis of protein expressions of p-Lyn and Lyn of mice; (F) mRNA level of Lyn of mice.
Fig 5: siRNA-lyn knockdown efficiency. (A) Representative images of protein expression of Lyn in cultured primary microglia after siRNA-lyn knockdown; (B) statistical analysis of protein expression of Lyn in cultured primary microglia after siRNA-lyn knockdown; (C) mRNA level of Lyn in cultured primary microglia after siRNA-lyn knockdown; (D) representative images of protein expression of Lyn of mice after siRNA-lyn knockdown; (E) statistical analysis of protein expressions of Lyn of mice after siRNA-lyn knockdown; (F) mRNA level of Lyn of mice after siRNA-lyn knockdown.
Supplier Page from Abcam for Anti-Lyn antibody